Kasius: Yo-Yo Rodriguez? How peculiar.
Yo-Yo: Says the space mime!

Sousa: Hey, what are you doing up?
Daisy: Why do you care?
Sousa: Because you don't.

The only power I got to worry about is horsepower.

Robbie

We'll discuss the consequences of your little science fair later.

Coulson

Deke: Okay, this is probably the part where I should explain--
Coulson: No, this is the part where May breaks your *face*! You explaining? Kinda secondary!

May: What's your name?
Nathanson: Nathanson, ma'am. What can I do?
May: Get lost, Nathanson.

Man: Welcome, sir. You are on our visitor list, but your secretary is not.
Coulson: Oh, she's not my secretary. She's my boss.
Simmons: I think you'll agree, I don't need to be on the list.
Man: Right. Sorry, ma'am. It's an honor. Uh, right this way Miss Carter.
Coulson: You're enjoying this way too much, Peggy.

Whatever you do, don't breathe. That was a joke.

Simmons

Gideon: So how long do we have once the portal reopens?
Mr. Giyera: 73 seconds.
Simmons: He's wrong. Your team's calculations are off at 61. 12 seconds might not seem like much, but on that planet it can be the difference between life and death.
Gideon: Thank you.
Simmons: I'm not doing it for you. Don't want you all mucking up Fitz's return.
Gideon: Did you notice the Hebrew word Maveth carved around the castle?
Simmons: Death.
Gideon: That's right, well death is the only thing returning through that portal. Fitz is just an offering to show it the door.

The people who put these ops together are the best of the best. They know what they're doing.

Coulson

I've been where you are right now so please believe me when I tell you you don't have to be afraid.

Coulson

Coulson: He must have blown his tracker.
Daisy: Yeah, because he's creeped out that you put a tracker in him.

Agents of S.H.I.E.L.D. Quotes

Kasius: Yo-Yo Rodriguez? How peculiar.
Yo-Yo: Says the space mime!

Sousa: Hey, what are you doing up?
Daisy: Why do you care?
Sousa: Because you don't.