Coulson: I don't know how else to explain it but it needs to be carved.
Skye: Does it just appear in your mind like some sort of vision?
Coulson: No, it just kinda happens. Which is really frustrating because no matter how much I carve it still means nothing to me. I don't even have a theory.
Skye: I do. It's a map.

Guess being a douchebag runs in the family.

Skye

She can handle it. Deception's her forte. I mean that sincerely, not passive-aggressively. As in it's a good attribute for a spy.

Hunter

Senator Ward: The American people are looking for a simple enemy Mr. Coulson. It's what makes them feel safe.
Coulson: And how do you think they'd feel if they knew your brother was HYDRA? That's an awfully complicated idea to grasp. Especially in an election year.
Senator Ward: Are you threatening me Mr. Coulson?
Coulson: No, I'm telling you I have your brother in my basement.

Mack: Look I know you two have a past but from what I've seen the only thing that makes him worse is you.
Simmons: I know, why do you think I left?

May gave specific instructions, you go on a date with a crazy wall I have to chaperone.

Skye

Skye: You two don't have a single thing in common unless you have some Enya albums you've been hiding.
Coulson: No, nothing in common at all.
Skye: I take that back.

Simmons: She had GH.325 in her blood.
Coulson: A T.A.H.I.T.I. patient?
Simmons: Whoever killed her also had GH.325 in their blood.

Fitz: He'll recover, he did before. Besides brains never delete files they just lose connections, but there's always a back up. It's just a matter of digging and finding them.
Mack: So you've got back up files too?

I was put in charge of the T.A.H.I.T.I. project so those people are my responsibility. If going back in this machine saves even one life, then it's worth the risk.

Coulson

Discovery requires experimentation and this experiment will take time.

Whitehall

Hill: What does S.H.I.E.L.D. stand for Agent Ward?
Ward: Strategic Homeland Intervention, Enforcement and Logistics Division.
Hill: And what does that mean to you?
Ward: That someone really wanted our initials to spell SHIELD.

Agents of S.H.I.E.L.D. Quotes

Kasius: Yo-Yo Rodriguez? How peculiar.
Yo-Yo: Says the space mime!

Sousa: Hey, what are you doing up?
Daisy: Why do you care?
Sousa: Because you don't.