Supernatural Season 6 Episode 16
...And Then There Were None
On this week's episode of Supernatural...
Sam and Dean seek out the Mother of All's newest formed creature, a parasite that enters through the human ear and takes control.
On their journey, they team up with Bobby, Rufus, Samuel, and Gwen.
Unfortunately during the search, the creature takes over Dean and shoots Gwen. Sam shoots Samuel because he thinks the parasite is inside. Finally, a parasite controlled Bobby kills Rufus.
After burying Rufus, the brothers agree to not let past problems get in the way of forgiveness. Family is family.