Sam and Dean are on the case in this Supernatural still. It's from the episode "There Will Be Blood."
Added:
It's Crowley! What Supernatural fan out there does not get excited when this character makes an appearance?
Added:

Supernatural Season 7 Episode 22 Quotes

Dean: Don't say pie.
Sam: Definitely pie.
Dean: Bastards.

Dean: Ok, I've read this more times than the Playboy I found in dad's duffel.
Sam: Anna Nicole?
Dean: Anna Nicole.