Ward: Where's your side arm?
May: If I need a gun, I'll take one.
Ward: Right, I forgot I was working with "The Calvary."
May: Don't ever call me that.

Skye: I don't even know where we are going.
Coulson: Peru. That's where the 084 was reported.
Skye: And an 0-8-4 is?
Coulson: Object of unknown origin, kind of like you. Team goes in determines if it's useful or poses a threat. Last one turned out to be pretty interesting.
Skye: What was the last one?
Coulson: A hammer.

Director Mace: In case you haven't noticed, Phil, we're not a team that trusts. We're not a team at all.
Coulson: You may be right. But we better become one if we're going to stop Eli Morrow from blowing up half of Los Angeles.

Doc: Tahiti. He really doesn't know does he?
Hill: He can never know.

Virgil: You’re here. Agent Coulson of SHIELD. You’ve come to save us!
Coulson: Just to clarify, where is “here,” who is “us,” and from what?!

Ward: A train that ?
Coulson: Yep, Vanished. Which I might add, is not easy.

Coulson: What are you doing?
Simmons: I’m getting into character. My undercover persona harbors some residual resentment to her absent American father.

This is the job, we have to make certain sacrifices. Like free time.

Bobbi

A team that trusts is a team that triumphs? That's a bit cheesy.

Mace [to Simmons]

Gideon: The Von Strucker family vault does not exist.
Ward: Truthfully I'd believe you, if you hadn't sacrificed the only heir to it. You control the vault now, right? The vault is said to have HYDRA's greatest power. Why don't you and I put it to good use?

Ward: You’re right, he’s Asgardian.
Coulson: Good thing. That would have been awfully embarrassing.

Skye: You guys might think its old news, but its new news to everyone else. Asgardians are aliens from another planet that visited us 1000 years ago.
Coulson: Or more
Skye: Because we couldn't understand aliens we thought they were gods?
Coulson: That’s where our Norse mythology comes from.

Agents of S.H.I.E.L.D. Quotes

Kasius: Yo-Yo Rodriguez? How peculiar.
Yo-Yo: Says the space mime!

Sousa: Hey, what are you doing up?
Daisy: Why do you care?
Sousa: Because you don't.