Coulson: I can’t think of a single time when anything alien in human hands ended well.
Skye: Wouldn't mind getting my human hands on Thor. He’s so dreamy.
Coulson: Sure he’s handsome but
May: No he’s dreamy.

As far as I'm concerned, you're just another redshirt, like so many others who tried to stop me from saving the world. So, cool original story, bro. But this means nothing to me.

Coulson [to Ivanov]

Yo-Yo: You won’t listen! I am in a nightmare, and I’m screaming at the top of my lungs and not one of you can hear me!
Simmons: We hear you!
Yo-Yo: But you won’t *listen*! You all saw what happened, and none of you will help me stop this! [turns to Mack] I’m alone. Even you, even you won’t help me.
Mack: No. You’re not alone. We’re in this with you, but you need to hold it together.
Yo-Yo: There’s nothing holding us together!
Mack: Yes, there is! HOPE! We never give up. We’re always held together by hope! So we cling to it, and we vote. We vote.

I’ve lived a life surrounded by heroes. None bigger than all of you.

Coulson

Coulson: What is it with the flowers?
Raina: Who doesn't like flowers? I'm glad you noticed.

You can't undo what's been done, that will be with you forever. But trying hold to this life, clinging to the person you thought could be, that's hell.

Melinda

Ward: So Director Fury faked your death to motivate the Avengers?
Hill: The death of a comrade is a particularly effective motivator.
Coulson: It wasn't that big of stretch, I stopped breathing for 40 seconds
Hill: That time gets longer each time you tell it.
Coulson: You get shanked by the Asgardian Mussolini and you can tell it your way.

Coulson: Skye, hey? Skye are you okay? Skye?
Daisy: It's Daisy.
Coulson: What?
Bobbi: It's Daisy now sir. You're really having a hard time with this huh?
Coulson: Damn it, yeah.

You filthy calamari Mata Hari.

Talbot [to Hale]

Enoch, this looks like the start of a marvelous friendship.

Ernest

Beaten to within an inch of her life. Nevertheless, she persisted.

Fitz [on Daisy]

Hill: For that you will have to ask Agent Coulson.
Ward: I'm clearance level 6, I know that Coulson died before the battle of New York,
Coulson: [steps out of shadow] Welcome to level 7. Sorry, that corner was really dark, I couldn't help myself. I think there's a bulb out.

Agents of S.H.I.E.L.D. Quotes

Kasius: Yo-Yo Rodriguez? How peculiar.
Yo-Yo: Says the space mime!

Sousa: Hey, what are you doing up?
Daisy: Why do you care?
Sousa: Because you don't.